⊃ bioreactors for cell therapies

notes from a recorded talk on bioreactors (the image above is a muscle bioreactor that stretches out / exercises the cells..pretty cool!)

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⊃ OTR and kLa

I’m reading this PDF from UGA on oxygen mass transfer..some notes:

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⊃ aseptic technique

Lots of great videos out there on aseptic handling, woo!

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⊃ design of experiments

a software tool, pyDOE some lecture notes on the topic and a video:

⊃ a five day fed batch protocol

interesting tidbits from a very detailed fed-batch protocol:

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⊃ DO probes

learning next about sensors for dissolved oxygen..

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⊃ pH probes

learning about pH probes..

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⊃ sterilizing instruments

reading about various ways to clean probes and instruments – wikipedia has a great review, as does Finesse.

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⊃ genedrive

notes from Hammond et. al’s paper in Nature Biotech: A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae and the Wyss lab’s gene drive FAQ.

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⊃ cell culture and automation

I’ve been reading some interesting papers on cell culture and automation, like this 2013 thesis on microbioreactors, published by Shireen Goh as her PhD work at MIT.

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